pHTS Reporter Vectors

Description

Kit Contents:

40µg of transfection grade plasmid.
40µg of transfection grade control plasmid.

Biomyx’ StimulightTM cis-reporting systems are designed for quick and convenient creation of stable cell lines to study in vivo activation of signal transduction pathways. The HSV TK promoter driven hygromycin or nemycin phosphotransferase expression cassette offers fast and efficient selection of stably transfected cells (with hygromycin or Geneticine®/G418, respectively). The cis-reporting vectors could also be used in transient assays before committing ones efforts to stable cell lines.

The StimulightTM cis-reporting system includes a group of vectors covers signal transduction pathways converging at several common enhancer elements including CRE (P2100, P2102, cyclic AMP response element), GAS (P2105, P2107, interferon g–activated sequence), ISRE (P2110, P2112, interferon-stimulated response element), AP1 (P2125, P2127, activator protein 1), NFAT (P2115, P2117, nuclear factor of activated T cells) and NFkB (P2120, P2122, binding sites for nuclear factor kB). Please refer to the product manual for sequences of enhancer elements.

A cloning vector (pHTS-MCS, Cat# P2130 with Hygromycin, and Cat# P2132 with Neomycin) with convenient cloning sites is also available so that one can create synthetic promoters to monitor new signaling pathways of interest. Some of the common features are:

¨ Hygromycin or neomycin resistance cassette for efficient stable cell selection

¨ Ready to use transfection purity plasmid provided

¨ Unique restriction site for linearization of vector improves stable cell selection efficiency

¨ High copy number plasmid for easy plasmid preparation

Product Details (pdf)